| Instructors | Frances E. Weaver, Ph.D. | Kevin Boyer, M.S. |
| Office | K 438 | K 512 |
| Phone | 499-4519 | 499-4010 |
| weaver@pop1.science.widener.edu | boyer@pop1.science.widener.edu | |
| Office Hours | T 11 a.m-12:15 p.m.,W 1-3 p.m.,TH 2-3:15 p.m., F 1-2 p.m. | posted on the door of K 512 |
Text: Benson, Harold J. Microbiological
Applications, complete version, 7th Ed. WCB/McGraw-Hill, Boston MA
1998
Materials: You must obtain a lab coat to be worn during
each lab session. You will also be assigned a microscope, lab
drawer and seat number. Students are responsible for the condition
of their assigned microscopes and lab drawers.
Rules: Follow the safeguards listed in the introduction on page VI (Introduction) of the lab manual. A handout describing how to aseptically handle bacterial cultures will also be distributed. These procedures must be observed during each session and are necessary to prevent the contamination of students as well as cultures. Attendance is mandatory for each lab session. Anyone who misses a lab for a non-verifiable reason will receive a grade of zero for any missed work. Legitimately missed labs may be made up at the instructor's perogative.
Grades: Laboratory grades will be determined by the following:
Lab Performance
15%
Unknown #1
25%
Unknown #2
30%
Final Exam
30%
The lab performance grade will be determined by attendance, lab reports, lab technique, and quizzes. The final will be a comprehensive multiple choice exam. The plus/minus grading system will be used. Final grades will be assigned based on your final average. The following scale will be used:
A 90-100
B 80-89
C 70-79
D 60-69
F <60
The Science Division strictly enforces the University’s policy on
academic fraud, as stated in Section F of the Student Handbook
Laboratory Schedule
| Lab | Date | Begin Exercise | Finish Exercise |
| 1 | Jan.20 | 1 Brightfield Microscopy
2,3 Darkfield & Phase-Contrast Microscopy (Demo) 6,9 Survey of Microorganisms |
|
| 2 | Jan. 27 | 8 Fungi (Handout)
11 Smear Preparation 12 Simple Stain |
9 |
| 3 | Feb. 3 | 10 Negative Stain
5 Microscopic Measurements 14 Gram Stain |
8 |
| 4 | Feb. 10 | 19 Motility Determination
Review Gram Stain |
14 |
| 5 | Feb. 17 | 20 Culture Media Prep. (Handout)
15 Endospore Stain |
19 |
| 6 | Feb. 24 | 21 Pure Culture Techniques
Review for Unknown |
20 |
| 7 | March 2 | UNKNOWN #1 | 21 |
| SPRING BREAK | March 9 | SPRING BREAK | SPRING BREAK |
| 8 | March 16 | 35 Temperature: Lethal Effects
39 Ultraviolet Light 41 Alcohol Evaluation 43 Antimicrobic Sensitivity Test 67 Bacterial Counts of Foods |
21 |
| 9 | March 23 | see next column | 35,39,41,43,67 |
|
10 |
March 30 | BEGIN UNKNOWN #2
45 Stock Cultures 46 Morphological Study 47 Cultural Characteristics 28 Bacterial Virus Cultivation (Handout) |
|
|
11 |
April 6 | 48 Biooxidations
49 Hydrolysis Reactions 50 Miscellaneous Tests 53 Enterotube |
45-47, 28 |
| 12 | April 13 | 51 Bergey's Manual
77 Staphylococci 78 Streptococci |
48-50, 53 |
| 13 | April 20 | 80 Urinary Tract Pathogens | 77,78 |
| 14 | April 27 | UNKNOWN #2 DUE
82 Slide Agglutination Test |
80 |