Instructor | Frances E. Weaver, Ph.D. | |
Office | K 438 | |
Phone | 499-4519 | |
weaver@pop1.science.widener.edu | ||
Office Hours | MWF 2:00-2:50, T 10-10:50 |
Text: Benson, Harold J. Microbiological
Applications, complete version, 8th Ed. WCB/McGraw-Hill, Boston MA
2002
Materials: You must obtain a lab coat to be worn during
each lab session. You will also be assigned a microscope, lab
drawer and seat number. Students are responsible for the condition
of their assigned microscopes and lab drawers.
Rules: Follow the safeguards listed in the introduction on page VI (Introduction) of the lab manual. A handout describing how to aseptically handle bacterial cultures will also be distributed. These procedures must be observed during each session and are necessary to prevent the contamination of students as well as cultures. Attendance is mandatory for each lab session. Anyone who misses a lab for a non-verifiable reason will receive a grade of zero for any missed work. Legitimately missed labs may be made up at the instructor's perogative.
Grades: Laboratory grades will be determined by the following:
Lab Performance
15%
Unknown #1
25%
Unknown #2
30%
Final Exam
30%
The lab performance grade will be determined by attendance, lab reports, lab technique, and quizzes. The final will be a comprehensive multiple choice exam. The plus/minus grading system will be used. Final grades will be assigned based on your final average. The following scale will be used:
A 90-100
B 80-89
C 70-79
D 60-69
F <60
The Science Division strictly enforces the University’s policy on
academic fraud, as stated in the Student Handbook, Academic Policies, Procedures
and Regulations; Section F.
Laboratory Schedule
Lab | Date | Begin Exercise | Finish Exercise |
1 | Jan.17 | 1 Brightfield Microscopy
2,3 Darkfield & Phase-Contrast Microscopy (Demo) 6,9 Survey of Microorganisms |
|
2 | Jan. 24 | 8 Aseptic Technique
10 Fungi (Handout) 12 Smear Preparation 13 Simple Stain |
9 |
3 | Jan. 31 | 11 Negative Stain
5 Microscopic Measurements 15 Gram Stain |
8,10 |
4 | Feb. 7 | 19 Motility Determination
Review Gram Stain |
15 |
5 | Feb. 14 | 20 Culture Media Prep. (Handout)
16 Endospore Stain |
19 |
6 | Feb. 21 | 21 Pure Culture Techniques
Review for Unknown |
20 |
7 | Feb 28 | UNKNOWN #1 | 21 |
SPRING BREAK | March 7 | SPRING BREAK | SPRING BREAK |
8 | March 14 | 35 Temperature: Lethal Effects
39 Ultraviolet Light 41 Alcohol Evaluation 43 Antimicrobic Sensitivity Test 69 Bacterial Counts of Foods |
21 |
9 | March 21 | see next column | 35,39,41,43,67 |
10 |
March 28 | BEGIN UNKNOWN #2
45 Stock Cultures 46 Morphological Study 47 Cultural Characteristics 28 Bacterial Virus Cultivation (Handout) |
|
11 |
April 4 | 48 Biooxidations
49 Hydrolysis Reactions 50 Miscellaneous Tests 53 Enterotube |
45-47, 28 |
12 | April 11 | 51 Bergey's Manual
78 Staphylococci 79 Streptococci |
48-50, 53 |
13 | April 18 | 81 Urinary Tract Pathogens | 78,79 |
14 | April 25 | UNKNOWN #2 DUE
83 Slide Agglutination Test |
81 |