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Campus Cruiser

     Image by Andre Karwath               

Main Research Interest:
Understanding the regulation of alternative splicing
using the model organism Drosophila melanogaster

The genome of the simple fruit fly contains approximately 15,000 genes while that of a more complex human contains only twice that with 30,000. This apparent discrepancy between gene number and complexity can be reconciled by considering alternative splicing, a universal biochemical mechanism that works similarly in diverse organisms from yeast to worms to insects to humans. The process of splicing, whether general or alternative, regulates gene expression and maximizes and the number of proteins that one gene can produce. With over 70% of human genes predicted to be alternatively spliced, many human diseases including cancer have been attributed to mutations in splice site selection signals or the splicing machinery itself. Yet little is known about the earliest and most critical steps of splicing regulation, when intron and exon borders or pre-mRNA are first recognized and defined by the spliceosome. Our lab takes advantage of the wide spectrum of genetic manipulations and biochemical analysis possible in flies to examine the function of proteins integral to tissue specific alternative splicing.

Detection of alternative splicing events between soma and testes by RT-PCR

 
Decrease the concentration of
 splicing factors
by RNAi knockdown
using tissue-specific
 gal4 drivers
and examine the effect on
alternative splicing events
 
 

Identify regulatory proteins
associated with the
 early spliceosome
in testes using a
 tagged splicing factor

Identification  of alternative splicing between males and females in the sexually dimorphic insect Stiphra sp. using the  Drosophila melanogaster sex determination pathway as a model